For 10 Groups (20 Experiments) >> Soil is a rich source for the isolation of various bacterial strains, but possible dangers exist in the isolation of bacteria that may prove to be a human pathogen. In the laboratory, samples of soils that are free of any environmental bacteria will be used. The sterile samples will contain one or two specific safe soil bacteria. Students will isolate and characterize the strains. With caution in the field, students will also isolate the various bacteria from soil samples. Store all components at room temperature. >> EXPERIMENT INCLUDES • Complete instructions, background information, and study questions • Biologicals and Gram’s Stains • ReadyPour™ Luria Broth Agar • Fluorescent reagents for bacterial detection • Petri plates • 10 ml pipets and transfer pipets • Inoculating loops • Sterile swabs >> REQUIREMENTS • Incubation Oven • Microwave, Hot Plate or Bunsen burners • Microscope with Oil Immersion Lens • Microscope Immersion Oil • Slides and Slide Holders • Laboratory Glassware • Squeeze Bottle • Bleach or Disinfectant • Trays for Disinfectant • Hot Gloves and Goggles • Latex or vinyl gloves • Distilled Water • 95% Ethanol •Toothpicks >> TIME REQUIREMENTS 2 or 3 lab periods with overnight incubations.