For 10 Groups >> Water pollution is a major worldwide problem and the quality of drinking water is vitally important to society. Poor treatment of human sewage and eutrophication (the over-enrichment of nutrients) leads to high levels of bacterial growth and pathogenicity. Students will test a control and simulated contaminated water samples using color and fluorescent reagents that detect bacteria. The experiments will serve as the basis for screening of water samples obtained from various water bodies in the environment. As an optional experiment students will plate the known bacteria and do a stain identification. All components can be stored at room temperature. >> EXPERIMENT INCLUDES • Complete instructions, background information, and study questions • Biologicals and Gram’s Stains • ReadyPour™ Luria Broth Agar • Fluorescent reagents for bacterial detection • Petri plates • 10 ml Pipet (sterile) • Inoculating loops • Transfer Pipets • Sterile Swabs • Microcentrifuge test tubes with caps >> REQUIREMENTS • Incubation Oven • UV Long Wave Lamp • Microwave, Hot Plate or Bunsen burners • Microscope with Oil Immersion Lens • Microscope Immersion Oil • Slides & Slide Holders • Laboratory Glassware • Squeeze Bottle • Bleach or Disinfectant • Trays for Disinfectant • Hot Gloves & Goggles • Latex or vinyl Gloves • Distilled Water • 95% Ethanol • Toothpicks >> TIME REQUIREMENTS >> Requires approx. 30 minutes and overnight incubation.